IPTG Solution 100 mM (Biotechnology Grade)
| Catalog number: | B2010137 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Volume/Weight: | 10 x 1 mL |
| pH: | 7 |
| Supplied as: | Ready-to-use solution |
| Appearance: | Clear solution |
| Applications: | optimized stock solution of IPTG (isopropyl-beta-D-thiogalactopyranoside), a synthetic analog of lactose. IPTG inactivates the lac repressor of the lac operon, trigeering the induction of beta-galactosidase synthesis. IPTG is used with X-Gal in the blue/white colony screening. it also used to induce the expression of recombinant protein under the control of the lac operon. |
| Storage: | -20C |
| Keywords: | IPTG solution, IPTG inducer, IPTG expression, IPTG X-Gal |
| Grade: | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered on a 0.22 um. |
References
- Tran DTM, Phan TTP, Huynh TK, Dang NTK, Huynh PTK, Nguyen TM, Truong TTT,
- Tran TL, Schumann W, Nguyen HD. Development of inducer-free expression plasmids
- based on IPTG-inducible promoters for Bacillus subtilis. Microb Cell Fact. 2017
- Jul 25;16(1):130.
- Marbach A, Bettenbrock K. lac operon induction in Escherichia coli:
- Systematic comparison of IPTG and TMG induction and influence of the
- transacetylase LacA. J Biotechnol. 2012 Jan;157(1):82-8.
