Thymidine Phosphorylase, Recombinant from Escherichia coli
| Catalog number: | B2019685 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 5000 units |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Solution |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | 2-8C |
| Keywords: | Thymidine:orthophosphate deoxy-D-ribosyltransferase |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Ge C, OuYang L, Ding Q, Ou L. Co-expression of recombinant nucleoside phosphorylase from Escherichia coli and its application Appl Biochem Biotechnol. 2009 Oct;159(1):168-77.
- Gbaj A, Edwards PN, Reigan P, Freeman S, Jaffar M, Douglas KT. Thymidine phosphorylase from Escherichia coli: tight-binding inhibitors as enzyme active-site titrants J Enzyme Inhib Med Chem. 2006 Feb;21(1):69-73.
- Karamitros CS, Somody CM, Agnello G, Rowlinson S. Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase Front Bioeng Biotechnol. 2021 Dec 17;9:793985.
- Moghaddam A, Bicknell R. Expression of platelet-derived endothelial cell growth factor in Escherichia coli and confirmation of its thymidine phosphorylase activity Biochemistry. 1992 Dec 8;31(48):12141-6.
- Ding Q, Ou L, Wei D, Wei X. Optimum induction of recombinant thymidine phosphorylase and its application Nucleosides Nucleotides Nucleic Acids. 2011 May;30(5):360-8.
- Carlson CA, Stewart GJ, Ingraham JL. Thymidine salvage in Pseudomonas stutzeri and Pseudomonas aeruginosa provided by heterologous expression of Escherichia coli thymidine kinase gene J Bacteriol. 1985 Jul;163(1):291-5.
- Moskaleva ND, Sukhodolets VV. [Escherichia coli K-12 mutants for the thymidine phosphorylase structural gene that retain the anabolic function of the enzyme] Genetika. 1981;17(9):1606-17.
- McNally VA, Gbaj A, Douglas KT, Stratford IJ, Jaffar M, Freeman S, Bryce RA. Identification of a novel class of inhibitor of human and Escherichia coli thymidine phosphorylase by in silico screening Bioorg Med Chem Lett. 2003 Nov 3;13(21):3705-9.
- Esipov RS, Gurevich AI, Chuvikovsky DV, Chupova LA, Muravyova TI, Miroshnikov AI. Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes Protein Expr Purif. 2002 Feb;24(1):56-60.
