Thermolysin from Geobacillus stearothermophilus
| Catalog number: | B2014172 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 5 mg |
| Molecular Weight or Concentration: | 30-350 units/mg protein |
| Supplied as: | Powder |
| Applications: | molecular tool for various biochemical applications |
| Storage: | -20C |
| Keywords: | Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Ke Q, Chen A, Minoda M, Yoshida H. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus Food Chem Toxicol. 2013 Sep;59:541-8.
- Ripp S, Turunen P, Minot ED, Rowan AE, Blank KG. Deciphering Design Principles of Frster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case ACS Omega. 2018 Apr 12;3(4):4148-4156.
- Amelunxen RE, Murdock AL. Mechanisms of thermophily CRC Crit Rev Microbiol. 1978;6(4):343-93.
- Naitow H, Matsuura Y, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Tanaka R, Tanaka T, Sugahara M, Kobayashi J, Nango E, Iwata S, Kunishima N. Protein-ligand complex structure from serial femtosecond crystallography using soaked thermolysin microcrystals and comparison with structures from synchrotron radiation Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):702-709.
- D_rrschmidt P, Mansfeld J, Ulbrich-Hofmann R. Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate Biophys Chem. 2010 Mar;147(1-2):66-73.
- Eijsink VG, Matthews BW, Vriend G. The role of calcium ions in the stability and instability of a thermolysin-like protease Protein Sci. 2011 Aug;20(8):1346-55.
- Mansfeld J, Petermann E, D_rrschmidt P, Ulbrich-Hofmann R. The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus Protein Expr Purif. 2005 Feb;39(2):219-28.
- Van den Burg B, Vriend G, Veltman OR, Venema G, Eijsink VG. Engineering an enzyme to resist boiling Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2056-60.
- Mansfeld J, Ulbrich-Hofmann R. The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents Biotechnol Bioeng. 2007 Jul 1;97(4):672-9.
