SCDase (Shewanella alga)
| Catalog number: | B2016769 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 0.25 U |
| Molecular Weight or Concentration: | 76.2 kDa |
| Supplied as: | Solution |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | -80C |
| Keywords: | Ricin-type -trefoil Lectin Domain Protein, Sphingolipid Ceramide N-deacylase |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Han YB, Wu L, Rich JR, Huang FT, Withers SG, Feng Y, Yang GY. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids Appl Microbiol Biotechnol. 2015 Aug;99(16):6715-26.
- Furusato M, Sueyoshi N, Mitsutake S, Sakaguchi K, Kita K, Okino N, Ichinose S, Omori A, Ito M. Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8 J Biol Chem. 2002 May 10;277(19):17300-7.
- Okino N, Ito M. Hydrolysis of glycosphingolipids by a sphingolipid ceramide N-deacylase (SCDase) 2021 Sep 28 [updated 2022 Mar 18]. In: Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021.
- Chakraberty R, Reiz B, Cairo CW. Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS Anal Biochem. 2021 Oct 15;631:114361.
- Tirodkar TS, Voelkel-Johnson C. Sphingolipids in apoptosis Exp Oncol. 2012 Oct;34(3):231-42.
- Tang W, Fang F, Liu K, Huang Z, Li H, Yin Y, Wang J, Wang G, Wei L, Ou Y, Wang Y. Aligned Biofunctional Electrospun PLGA-LysoGM1 Scaffold for Traumatic Brain Injury Repair ACS Biomater Sci Eng. 2020 Apr 13;6(4):2209-2218.
- Okino N, Ito M. Simultaneous quantification of glucosylceramide and galactosylceramide by high-performance liquid chromatography using o-phtalaldehyde derivatives 2021 Oct 11 [updated 2022 Mar 21]. In: Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021.
- Cho KW. Bioluminescent assay for sphingolipid ceramide N-deacylase using Vibrio harveyi dark mutant M-17 J Microbiol. 2008 Oct;46(5):585-9.
- Li Y, Arigi E, Eichert H, Levery SB. Mass spectrometry of fluorocarbon-labeled glycosphingolipids J Mass Spectrom. 2010 May;45(5):504-19.
