Intrinsic Factor ABEI Conjugate

Intrinsic Factor ABEI Conjugate Catalog number: B2010619 (0.1 mg)

Intrinsic Factor ABEI Conjugate is a biotechnology-grade conjugate of porcine intrinsic factor (IF) with N-(4-Aminobutyl)-N-ethylisoluminol (ABEI), supplied as a solution in a defined 0.1 mg amount at 1 mg/mL. As specified by MD, it is intended as a versatile molecule for research on vitamin B12 metabolism and for the development of vitamin B12 assays. The ABEI label provides chemiluminescent capability in compatible detection systems, supporting construction of CLIA-style methods and related light-emitting readouts. Storage at 20C aligns with routine handling of ABEI-tagged proteins.

Because intrinsic factor binds cobalamin with high specificity, ABEI-conjugated IF can act as a functional component in competitive or sandwich assay architectures, serve as a tracer/capture element, or support immobilization on streptavidin-free platforms that detect ABEI chemiluminescence directly. The ready-to-use solution format removes conjugation and reconstitution steps, while the defined concentration simplifies titration and cross-platform transfer during method development. The biotech-grade preparation (Type I water, 0.22 m filtration) helps minimize background and contributes to reproducible signal characteristics across runs and lots.

Typical uses include building vitamin B12 calibration systems, validating binding and wash conditions, or prototyping assay layouts for research applications. Overall, this product offers a practical, high-purity option for teams standardizing vitamin B12 assay components and pursuing mechanistic studies of IFcobalamin interactions.

Why researchers choose this product:

• Defined 0.1 mg amount at 1 mg/mL supports predictable assay setup
• ABEI label provides chemiluminescent detection capability
• Ready-to-use solution; no in-house conjugation required
• Specified storage at 20C for stability
• Biotechnology-grade preparation (Type I water, 0.22 m filtration) for low background

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Batt RM, Horadagoda NU, McLean L, Morton DB, Simpson KW. Identification and characterization of a pancreatic intrinsic factor in the dog. Am J Physiol. 1989 Mar;256(3 Pt 1):G517-23.
• 2: Meikle DD, Bull J, Callender ST, Truelove SC. Intrinsic factor secretion after vagotomy. Br J Surg. 1977 Nov;64(11):795-7.
• 3: TRINCAO C. O factor intrnseco [The intrinsic factor]. An Inst Med Trop (Lisb). 1957 Mar-Jun;14(1-2):221-33.
• 4: Marcoullis G, Merivuori H, Grsbeck R. Comparative studies on intrinsic factor and cobalophilin in different parts of the gastrointestinal tract of the pig. Biochem J. 1978 Sep 1;173(3):705-12.
• 5: Herbert V, Herzlich B. Quantitation of intrinsic factor. Blood. 1983 Apr;61(4):819.
• 6: Meeroff JC, Zagalsky D, Meeroff M. Intrinsic factor deficiency in adults with normal hydrochloric acid production. Gastroenterology. 1981 Mar;80(3):575-7.
• 7: Festen HP. Fysiologie en pathofysiologie van de intrinsic factor-secretie en de cobalamine(vitamine B12)-absorptie [Physiology and pathophysiology of intrinsic factor secretion and cobalamin (vitamin B12) absorption]. Ned Tijdschr Geneeskd. 1992 Sep 19;136(38):1851-6.
• 8: Waters HM, Dawson DW. An enzyme immunoassay for intrinsic factor in urine. Clin Lab Haematol. 1999 Jun;21(3):169-72.
• 9: LATNER AL. The intrinsic factor. Biochem J. 1955 Feb 19;59(337th Meeting):xxviii-xxix.
• 10: Cotter R, Rothenberg SP. Solubilization, partial purification and radioassay for the intrinsic factor receptor from the ileal mucosa. Br J Haematol. 1976 Nov;34(3):477-87. pubmed.ncbi.nlm.nih.gov