Glutamate Dehydrogenase (Bovine Liver)

Glutamate Dehydrogenase (Bovine Liver)

Glutamate Dehydrogenase (GLDH) from bovine liver is a mitochondrial enzyme that catalyzes the oxidative deamination of L-glutamate to -ketoglutarate, a key intermediate in the Krebs cycle. This product is supplied as a freeze-dried, ready-to-use powder with a specific activity of 19 U/mg and a minimum purity of 99%. It is ideal for applications in metabolic pathway analysis, enzymatic assays, and studies of nitrogen homeostasis and neurochemistry.

GLDH plays a pivotal role at the intersection of amino acid metabolism and energy production, making it a valuable tool for research in biochemistry, oncology, and neurobiology. The enzyme is stable at 20C and should be reconstituted in sterile buffer for optimal performance. All components are prepared using Type I ultrapure water and filtered through 0.22 m membranes to ensure consistency and reproducibility across batches.

Key benefits:

• High-purity mitochondrial enzyme from bovine liver
• Supports studies in amino acid metabolism and oxidative deamination
• Validated specific activity (19 U/mg)
• Lyophilized format for long-term stability
• Ideal for neurochemical, metabolic, and enzymatic research

Indication for Use:
This product is for Research Use Only (RUO). It is not intended for human or animal use, diagnostic procedures, or therapeutic applications.

References:

• Smith HQ et al. GLDH at a metabolic branch point. Neurochem Res. 2019;44(1):117132.
• Craze ML et al. GLUD1 in breast cancer. Breast Cancer Res Treat. 2019;174(1):7991.
• Jin Y et al. Ebselen inhibition of GLDH. Assay Drug Dev Technol. 2018;16(2):115122.
• Li M et al. GLDH structure and insulin regulation. Neurochem Res. 2014;39(3):433445.
• Plaitakis A et al. GLDH deregulation in neurologic disorders. J Neurosci Res. 2013;91(8):10071017.
• Spanaki C et al. GLDH in ammonia metabolism. Neurotox Res. 2012;21(1):117127.
• Hull J et al. GLDH distribution in human brain. Neurochem Int. 2018;112:4958.
• Shashidharan P et al. Discovery of GLUD2. Neurochem Res. 2014;39(3):460470.
• Tomita T et al. Crystal structure of GLDH complex. FEBS Lett. 2017;591(11):16111622.
• Smith TJ et al. Allosteric regulation of GLDH. Trends Biochem Sci. 2008;33(11):557564.
• Nassar OM et al. Allosteric discrimination at NADH/ADP site. Protein Sci. 2019;28(12):20802088.