Cryptdin-4 (Mouse)
| Catalog | MDP0853 |
| CAS | 100742-61-8 |
| Amount | 100 g |
| Molecular Weight | 3755.2 g/mol |
| Sequence | Gly-Leu-Leu-Cys-Tyr-Cys-Arg-Lys-Gly-His-Cys-Lys-Arg-Gly-Glu-Arg-Val-Arg-Gly-Thr-Cys-Gly-Ile-Arg-Phe-Leu-Tyr-Cys-Cys-Pro-Arg-Arg Disulfide bonds: Cys4Cys29, Cys6Cys21, Cys11Cys28 |
| Supplied as | Powder |
| Storage | -20C |
| Applications | Molecular tool for biochemical research |
| Grade | Biotechnology grade |
Cryptdin-4 (Mouse) is a biotechnology-grade alpha-defensin peptide derived from Paneth cells in the murine small intestine. With a molecular weight of 3755.2 g/mol and a stabilized disulfide array, this peptide plays a key role in innate immunity and antimicrobial defense. Its structure enables selective bactericidal activity, particularly against noncommensal organisms, making it a valuable tool for host-pathogen interaction studies.
Supplied as a 100 g powder aliquot and stable at -20C, Cryptdin-4 is ideal for structural biology, peptide-lipid interaction assays, and functional analysis of defensin charge and folding. All components are highly pure and RUO-compliantthis product is not intended for human or animal use.
Key benefits:
- Biotechnology-grade purity for reproducible in vitro assays
- Defined disulfide array for structural and functional studies
- Validated in antimicrobial, folding, and peptide-lipid interaction research
- Stable at -20C; powder format for flexible reconstitution
- RUO-compliant: not for clinical or veterinary use
Indication for Use:
This product is for Research Use Only (RUO). It is not intended for human or animal use, diagnostic procedures, or therapeutic applications.
References:
- Jing W, Hunter HN, Tanabe H, Ouellette AJ, Vogel HJ. Solution structure of cryptdin-4, a mouse paneth cell alpha-defensin. Biochemistry. 2004 Dec 21;43(50):15759-66.
- Masuda K, Sakai N, Nakamura K, Yoshioka S, Ayabe T. Bactericidal activity of mouse -defensin cryptdin-4 predominantly affects noncommensal bacteria. J Innate Immun. 2011;3(3):315-26.
- Tanabe H, Qu X, Weeks CS, Cummings JE, Kolusheva S, Walsh KB, Jelinek R, Vanderlick TK, Selsted ME, Ouellette AJ. Structure-activity determinants in paneth cell alpha-defensins: loss-of-function in mouse cryptdin-4 by charge-reversal at arginine residue positions. J Biol Chem. 2004 Mar 19;279(12):11976-83.
- Rosengren KJ, Daly NL, Fornander LM, Jnsson LM, Shirafuji Y, Qu X, Vogel HJ, Ouellette AJ, Craik DJ. Structural and functional characterization of the conserved salt bridge in mammalian paneth cell alpha-defensins: solution structures of mouse CRYPTDIN-4 and (E15D)-CRYPTDIN-4. J Biol Chem. 2006 Sep 22;281(38):28068-78.
- Tomisawa S, Sato Y, Kamiya M, Kumaki Y, Kikukawa T, Kawano K, Demura M, Nakamura K, Ayabe T, Aizawa T. Efficient production of a correctly folded mouse -defensin, cryptdin-4, by refolding during inclusion body solubilization. Protein Expr Purif. 2015 Aug;112:21-8.
- Mishra A, Tai KP, Schmidt NW, Ouellette AJ, Wong GC. Small-angle X-ray scattering studies of peptide-lipid interactions using the mouse paneth cell -defensin cryptdin-4. Methods Enzymol. 2011;492:127-49.
- Maemoto A, Qu X, Rosengren KJ, Tanabe H, Henschen-Edman A, Craik DJ, Ouellette AJ. Functional analysis of the alpha-defensin disulfide array in mouse cryptdin-4. J Biol Chem. 2004 Oct 15;279(42):44188-96.
- Li Q, Zhang Z, Chen X, Wang B, Zhou Z, Yan L, Jiang J. [The change of cryptdin-4 mRNA expression in the intestine of mouse with experimental pancreatitis]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jan;34(1):92-4.
- Figueredo SM, Weeks CS, Young SK, Ouellette AJ. Anionic amino acids near the pro-alpha-defensin N terminus mediate inhibition of bactericidal activity in mouse pro-cryptdin-4. J Biol Chem. 2009 Mar 13;284(11):6826-31.
- Lngst N, Adler J, Kuret A, Peter A, Ruth P, Boldt K, Lukowski R. Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE(-/-) Mouse Model. Cells. 2022 Apr 17;11(8):1364.
