Coenzyme A Trilithium Salt

Coenzyme A Trilithium Salt Research Use Only

Coenzyme A Trilithium Salt is a high-purity activated form of Coenzyme A supplied as a 5 mg powder (MW 821.36 g/mol). This essential metabolic cofactor participates in numerous biochemical reactions, including fatty acid -oxidation, the citric acid cycle, and the biosynthesis of acetylcholine, fatty acids, and polyketides. It is widely used in enzymatic assays, metabolic studies, and as a substrate for acetyl-CoA synthetase and other CoA-dependent enzymes.

Scientific Overview

Coenzyme A (CoA) is a central cofactor in metabolism, functioning as an acyl group carrier in the form of acetyl-CoA, succinyl-CoA, and other acyl-CoAs. The trilithium salt form provides high solubility and stability for in vitro enzymatic reactions. CoA is indispensable for the citric acid cycle, -oxidation of fatty acids, cholesterol synthesis, and the production of acetylcholine and other neurotransmitters.

This Coenzyme A Trilithium Salt is suitable for:

• In vitro reconstitution of CoA-dependent enzymatic reactions
• Generation of acyl-CoA thioesters for metabolic studies
• Investigation of fatty acid oxidation and energy metabolism pathways
• Acetyl-CoA synthetase and other CoA-utilizing enzyme assays
• Biochemical research involving mitochondrial function and lipid metabolism

Usage & Handling Guidance

Store the lyophilized powder at -20 C in a desiccated environment. Reconstitute in ultrapure water or appropriate buffer immediately before use. Prepare fresh working solutions or single-use aliquots, as CoA is labile in solution. Keep on ice during experimental procedures.

• Recommended applications: Metabolic enzyme assays, acyl-CoA synthesis, and biochemical pathway studies
• Working concentration: Typically 0.11 mM final concentration (optimize for specific assay)
• Solubility: Highly soluble in water; prepare stock solutions as needed
• Handling: Protect from moisture and prolonged exposure to room temperature or alkaline conditions

What You Get

• 5 mg Coenzyme A Trilithium Salt as a high-purity powder
• Molecular weight 821.36 g/mol
• Ready for reconstitution in CoA-dependent enzymatic and metabolic assays
• Biotechnology-grade cofactor manufactured under strict quality controls
• For research use only (RUO)

Why Researchers Choose It

• High-purity Coenzyme A in stable trilithium salt form
• Essential cofactor for fatty acid metabolism, TCA cycle, and acetyl-CoA dependent reactions
• Ideal for in vitro enzyme kinetics and metabolic pathway reconstitution
• Convenient small-quantity powder for precise experimental use
• Manufactured under strict biotechnology-grade standards

Frequently Asked Questions (FAQ)

• What is the role of Coenzyme A?
  CoA acts as an acyl group carrier in central metabolism, forming acetyl-CoA, succinyl-CoA, and other thioesters essential for the citric acid cycle and fatty acid oxidation.
• How should I reconstitute the powder?
  Reconstitute in ultrapure water or neutral buffer immediately before use. Prepare fresh solutions or aliquots to maintain stability.
• What is the recommended working concentration?
  Typically 0.11 mM final concentration in enzymatic assays (optimize for your specific reaction).
• Is this product stable in solution?
  CoA is labile in solution; use freshly prepared stocks and keep on ice during experiments.
• Can this be used to generate acetyl-CoA?
  Yes. It is commonly used with acetyl-CoA synthetase or other enzymes to produce acyl-CoA thioesters in vitro.

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

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