Alkaline Phosphatase Assay Buffer (10X)
| Catalog number: | B2010944 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 50 mL |
| Molecular Weight or Concentration: | na |
| Supplied as: | Solution |
| Applications: | molecular tool for various biochemical applications |
| Storage: | -20C |
| Keywords: | AP Assay Buffer |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Sun J, Zhao J, Bao X, Wang Q, Yang X. Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol Anal Chem. 2018 May 15;90(10):6339-6345.
- Olczak M, Szulc B. Modified secreted alkaline phosphatase as an improved reporter protein for N-glycosylation analysis PLoS One. 2021 May 25;16(5):e0251805.
- Gong H, Little G, Cradduck M, Draney DR, Padhye N, Olive DM. Alkaline phosphatase assay using a near-infrared fluorescent substrate merocyanine 700 phosphate Talanta. 2011 May 15;84(3):941-6.
- Takayanagi T, Mine M, Mizuguchi H. Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase Anal Sci. 2020 Jul 10;36(7):829-834.
- Obayashi Y, Iino R, Noji H. A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate Analyst. 2015 Aug 7;140(15):5065-73.
- Harbron S, Eggelte HJ, Fisher M, Rabin BR. Amplified assay of alkaline phosphatase using flavin-adenine dinucleotide phosphate as substrate Anal Biochem. 1992 Oct;206(1):119-24.
- Schumann G, Dominick HC, Hellmann D, Klauke R, Mckesch M, Stekel H, von Schenck H, Kraft M, Nagel R, Hnseler E. Alkaline phosphatase activity: new assay for the Reflotron system. Results of the evaluation in eight clinical laboratories Clin Chem Lab Med. 2001 Jan;39(1):71-8.
- Lewandrowski K, Lee-Lewandrowski E, Bowers GN Jr, McComb RB. Investigation of N-methyl-D-glucamine buffer for assay of alkaline phosphatase in serum Clin Chem. 1992 Nov;38(11):2286-94.
- Khatun S, Biswas S, Mahanta AK, Joseph MM, Vidyalekshmi MS, Podder A, Maiti P, Maiti KK, Bhuniya S. Biocompatible fluorescent probe for detecting mitochondrial alkaline phosphatase activity in live cells J Photochem Photobiol B. 2020 Nov;212:112043.
