5,5-Dithiobis(2-nitrobenzoic Acid) DTNB
| Catalog number: | B2010677 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 1 g |
| Molecular Weight or Concentration: | 396.35 g/mol |
| Supplied as: | Powder |
| Applications: | molecular tool for the study of thiol-containing molecules |
| Storage: | RT |
| Keywords: | DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide, Ellmans Reagent, 3-Carboxy-4-nitrophenyl disulfide, 6,6-Dinitro-3,3-dithiodibenzoic acid |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Owen JB, Butterfield DA. Measurement of oxidized/reduced glutathione ratio Methods Mol Biol. 2010;648:269-77.
- Sullivan ML, Bonawitz ND. Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5-dithio-bis-[2-nitrobenzoic acid]) Plant Sci. 2018 Apr;269:148-152.
- Xing B, Shi Y, Tang W. Kinetic studies of the reactions of some metal reconstituted metallothioneins with the electrophilic disulfide 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) Biometals. 2000 Dec;13(4):295-300.
- Lara HH, Ixtepan-Turrent L, Garza-Trevio EN, Flores-Tevio SM, Borkow G, Rodriguez-Padilla C. Antiviral propierties of 5,5-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1 Virol J. 2011 Mar 24;8:137.
- Huang BH, Shen SS, Wei N, Guo XF, Wang H. Fluorescence biosensor based on silicon quantum dots and 5,5-dithiobis-(2-nitrobenzoic acid) for thiols in living cells Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 15;229:117972.
- Takeda K, Shigemura A, Hamada S, Gu W, Fang D, Sasa K, Hachiya K. Dependence of reaction rate of 5,5-dithiobis-(2-nitrobenzoic acid) to free sulfhydryl groups of bovine serum albumin and ovalbumin on the protein conformations J Protein Chem. 1992 Apr;11(2):187-92.
- Reithmeier RA. Inhibition of anion transport in human red blood cells by 5,5-dithiobis(2-nitrobenzoic acid) Biochim Biophys Acta. 1983 Jul 13;732(1):122-5.
- Li ZG. Quantification of hydrogen sulfide concentration using methylene blue and 5,5-dithiobis(2-nitrobenzoic acid) methods in plants Methods Enzymol. 2015;554:101-10.
- Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid J Pharm Biomed Anal. 2008 Dec 15;48(5):1375-80.
